Preclinical MRI Center
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Preclinical MRI Center
The Preclinical MRI Center (PMC) at the Charité – Virchow Campus provides state-of-the-art MRI services for preclinical models. Set up as a Charité Shared Facility, the PMC covers all steps necessary to include MRI phenotyping in preclinical studies. Both cardiac and non-cardiac applications are supported. Its location on the same floor as the Berlin Experimental Radionuclide Imaging Center (BERIC) of the Charité facilitates multi-modal imag-ing studies combining high-resolution MRI with latest-generation PET and SPECT imaging. Within the German Center for Cardiovascular Research (DZHK), the PMC offers its services as part of the Shared Expertise program (#SE135).
Services
- Support with study design and choice of imaging methods
- Advice on study applications
- Administrative and organizational support regard-ing animal housing and animal care
- Models of myocardial ischemia/ reperfusion, myocardial inflammation (EAM), pressure-induced heart failure (TAC)
- Adaptation and optimization of tailored MR acqui-sition strategies
- MR scanning
- Data handling
- Data analysis
Facilities
The PMC hosts a new-generation dedicated small-animal MRI system (MRsolutions MRS3017). Installed in early summer 2015, the system is equipped with high-performance gradient systems allowing advanced acquisition strategies. Operating at translational field strength (3 Tesla) in a "desktop MRI" design, the system provides similar contrast characteristics as clinical MR systems, enabling direct comparisons between clinical and preclinical imaging findings. Thanks to the full range of coils and state-of-the-art gating and monitoring devices, all body regions of both mice and rats, including the heart, can be studied in wide ranging applications.
Ongoing Trials
Distribution of novel iron particles for tumor therapy (industry sponsored)
In-vitro localization of renal stem-cell implants (Charité/ Berlin-Brandenburg Center of Regenera-tive Therapies, BCRT)
Myocardial remodeling and fibrosis in acute heart failure (industry sponsored)
Myocardial injury in experimental autoimmune myocarditis (Charité/German Heart Institute Berlin)
Key Publications
Jeuthe S, Wassilew K, O H-Ici D, da Silva TF, Münch F, Berger F, Kuehne T, Pieske B, Messrogh-li DR. Myocardial T1 maps reflect histological find-ings in acute and chronic stages of myocarditis in a rat model. J Cardiovasc Magn Reson. 2016 Apr 16;18:19.
O H-Ici D, Jeuthe S, Dietrich T, Berger F, Kuehne T, Kozerke S, Messroghli DR.
Closed-chest small animal model to study myocar-dial infarction in an MRI environment in real time. Int J Cardiovasc Imaging. 2015 Jan;31(1):115-21.
Schmerler P, Jeuthe S, O h-Ici D, Wassilew K, Lau-er D, Kaschina E, Kintscher U, Müller S, Muench F, Kuehne T, Berger F, Unger T, Steckelings UM, *Paulis L, *Messroghli D. Mortality and morbidity in different immunization protocols for experimental autoimmune myocarditis in rats. Acta Physiol (Oxf). 2014;210(4):889-98.
Jeuthe S, O H-Ici D, Kemnitz U, Dietrich T, Schnackenburg B, Berger F, Kuehne T, Messroghli D. Assessment of cardiac function and myocardial morphology using small animal Look-Locker inver-sion recovery (SALLI) MRI in rats. J Vis Exp. 2013;(77).
*Messroghli DR, *Nordmeyer S, Dietrich T, Dirsch O, Kaschina E, Savvatis K, O h-Ici D, Klein C, Ber-ger F, Kuehne T.
Assessment of diffuse myocar-dial fibrosis in rats using small animal Look-Locker inversion recovery (SALLI) T1 mapping.
Circ Cardiovasc Imaging 2011;4(6):636-40.
Messroghli DR, Nordmeyer S, Buehrer M, Kozerke S, Dietrich T, Kaschina E, Hucko T, Berger F, Klein C, Kuehne T. Small Animal Look-Locker Inver-sion recovery (SALLI) for simultaneous generation of cardiac T1 maps, cine, and IR-prepared images at high heart rates: Initial experience. Radiology 2011; 261(1):258-265.